诱变选育具有磺胺胍抗性的L-色氨酸产生菌的研究

采用紫外线、甲基磺酸乙酯(EMS)及半导体激光诱变的方法,处理产色氨酸谷氨酸棒状杆菌GA22(Phe-+Tyr-+5MTr)选育磺胺胍抗性菌株以提高L-色氨酸的产量。用紫外照射20S、EMS处理40 min及半导体激光辐照16 min,筛选得到抗性突变株GA507(Phe-+Tyr-+5MTr+SGr),产色氨酸的量达到5.35 g/L,较出发菌株提高50.3%,并具有良好的遗传稳定性。

Study on mutagenesis breeding of L-tryptophan producing strains with sulfonamide resistance method

A sulfonamide resistant mutant was obtained from Corynebacterium glutamicurn GA22 （Phe^- ＋ Tyr^- ＋ 5MT^r）. The strain producing L-tryptophan was irradiated respectively by UV, EMS and LD-laser to increase L-tryptophan production. The results indicated that the cells were treated by UV for 20 seconds, EMS for 40 minutes and then by LD-laser for 16 minutes, and A L-tryptophan highly producing mutant GA507 （Phe^- ＋ Tyr^- ＋ 5MT^r ＋ SGr ） was obtained. The L-tryptophan yield reached 5.35 g/L, which was 50. 3% higher than those of the parent strains. The mutant had better hereditary stability.