幽门螺杆菌Lpp20蛋白的生物信息学分析

目的：分析幽门螺杆菌Lpp20蛋白的主要化学和免疫学分子特征,为基因工程疫苗和诊断抗原的研究奠定基础。方法：根据Lpp20蛋白的氨基酸序列,应用生物信息学工具分析其蛋白序列,预测其信号肽、跨膜区、疏水性、二级结构、三级结构等性质。结果：Lpp20蛋白具有一段信号肽、脂蛋白信号肽酶切位点及脂盒模体,没有跨膜区,可能是一个外周膜蛋白;Lpp20蛋白的二级结构以α螺旋为主,其三级结构为一个致密的球状。结论：为基于幽门螺杆菌Lpp20蛋白的疫苗开发打下了基础。

Bioinformatics Analysis on Lpp20 Protein of Helicobacter pylori

Objective： To analyze and predict the physical and chemical characteristics of Lpp20 protein of Helicobacter pylori, and to provide some evidence for further research on antagonistic peptides＇ structures and functions. Methods： The sequence of Lpp20 was analyzed and predicted by the tools of bioinformaties in the following aspects： signal peptides, transmembrane topological structures, hydrophobicity or hydrophilicity, secondary and tertiary structure of protein. The potential B-cell epitopes were also predicted. Results： Lpp20 protein included a 24-aa signal peptide, and contained a cleavage site for lipoprotein signal peptide and a lipbox motif. The protein had no transmembrane domain, and may be a peripheral membrane protein. The predicted secondary structure of Lpp20 contained mostly a-helical conformation. The three-dimensional model of Lpp20 indicated that the protein folded as a compact and globular domain, and might be soluble. Conclusion： Our research established foundation for H.pylori vaccine preparation by using Lpp20 protein.