| 改良CTAB法提取番石榴叶片总DNA |
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| 引用本文： | 王家保,杜中军,雷新涛,徐碧玉.改良CTAB法提取番石榴叶片总DNA[J].生物技术通讯,2006,17(5):757-759. |
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| 作者姓名： | 王家保 杜中军 雷新涛 徐碧玉 |
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| 作者单位： | 1. 中国热带农业科学院,热带生物技术研究所,热带作物生物技术国家重点实验室,海南,海口,571101
2. 中国热带农业科学院,南亚热带作物研究所,广东,湛江,524091 |
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| 基金项目： | 科技部科技基础性工作和社会公益研究专项面上项目 |
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| 摘 要： | 目的:从番石榴叶片中快速提取高质量的总DNA。方法:改良CTAB法。主要改进之处在于不用液氮,而是直接研磨硅胶干燥样品;用高浓度CTAB、低浓度乙醇与NaCl盐析相结合等方法去除多糖。结果:应用改良后的方法可以快速提取番石榴叶片总DNA,有效去除组织中的多糖、蛋白质,抑制提取过程中的组织褐变。提取的DNA可用于限制性内切酶酶切和PCR扩增。结论:传统CTAB法经过改良,可用于快速提取番石榴高质量DNA。
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| 关 键 词： | 番石榴 DNA 提取 CTAB |
| 文章编号： | 1009-0002(2006)05-0757-03 |
| 收稿时间： | 12 2 2005 12:00AM |
| 修稿时间： | 2005年12月2日 |
Extracting Total DNA from Guava(Psidium guajava L.) Leaves by Improved CTAB Method |
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| WABG Jia-bao,DU Zhong-jun,LEI Xin-tao,XU Bi-yu.Extracting Total DNA from Guava(Psidium guajava L.) Leaves by Improved CTAB Method[J].Letters in Biotechnology,2006,17(5):757-759. |
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| Authors: | WABG Jia-bao DU Zhong-jun LEI Xin-tao XU Bi-yu |
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| Institution: | 1. State Key Laboratory of Biotchnology for Tropical Crops, Institute of Tropical Biotechnology, Haikou 571101; 2. Institute of Southern Subtropical Crops, Zhanjiang 524091; Chinese Academy of Tropical Agriculture Sciences; China |
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| Abstract: | Objective: To extract high quality total genomic DNA quickly from guava leaves. Methods: The improved CTAB DNA extraction method. The silicon gel-dried guava leaves were ground in the dry mortar without liquid N2. The polysaccharide was got rid of by combined use of high concentration of CTAB, low concentration of ethanol and high concentration of NaCl. Results: Using the improved CTAB method, total DNA could be isolated from guava leaves quickly. The polysachrides and protein in the leaves could be got rid of efficiently and the browning of tissues could be inhibited during the extraction process. DNA extracted by the method could be digested by restriction enzymes and used as template for PCR. Conclusion: The improved CTAB method could be used for isolating total DNA with high quality from guava leaves. |
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| Keywords: | guava (Psidium guajava L ) DNA extraction method CTAB |
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