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Immobilized mammalian cell cultivation in hollow fiber bioreactors |
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| Authors: | Piret J M Cooney C L |
| Affiliation: | a Biotechnology Laboratory and Department of Chemical Engineering, University of British Columbia, Vancouver, BC, Canada V6T 1W5 b Biotechnology Process Engineering Center, Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, U.S.A. |
| Abstract: | Cultivation of animal cells for the production of recombinant proteins is an important method for manufacturing complex proteins requiring posttranslational processing. One of the often considered methods for cultivation is by immobilization of the cells in hollow fiber bioreactors (HFBRs). These systems allow the cells to grow to high densities in a shear protected environment; furthermore the product can be accumulated in high concentration in the case of ultrafiltration HFBRs. Operation and scale-up are constrained by nutrient and product transport with oxygen transfer to growing cells being the most critical parameter. Mathematical models describing HFBRs have proved to be useful in quantitating and understanding the constraints and guiding the scale-up of this approach to animal cell cultivation. |
| Keywords: | Mammalian cell hollow fiber immobilized bioreactors metabolite gradients tissue capillary |
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