High-throughput DNA extraction from forest trees |
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| Authors: | Mervyn Shepherd Michael Cross Rhonda L. Stokoe Leon J. Scott Megan E. Jones |
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| Affiliation: | (1) Cooperative Research Centre for Sustainable Production Forestry, Centre for Plant Conservation Genetics, Southern Cross University, Military Rd, 2480 Lismore, New South Wales, Australia;(2) Centre for Plant Conservation Genetics, Southern Cross University, Military Rd, 2480 Lismore, New South Wales, Australia |
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| Abstract: | ![]()
It is difficult to extract pure high-quality DNA from trees, which may not be amenable to advances in extraction methods suitable for other plants. A new commercial high-throughput DNA extraction system, using a silica binding matrix for purification and a multisample mixer mill for tissue disruption, was evaluated for its suitability withEucalyptus spp.,Pinus spp., andAraucaria cunninghamii (hoop pine). DNA suitable for a range of molecular biology applications was successfully extracted from all genera. The method was highly reliable when tested in more than 500 preparations and could be adapted to different tree species with relatively minor modifications. |
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| Keywords: | AFLP Araucaria Eucalyptus microsatellite Pinus |
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