Elutriation of human keratinocytes and melanocytes fromin vitro cultured epithelium |
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Authors: | Fiorella D'Anna Michele De Luca Ranieri Cancedda Antonio Zicca Adriano T. Franzi |
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Affiliation: | (1) Istituto di Anatomia Umana Normale, Universitá di Genova, Italy;(2) Istituto Nazionale per la Ricerca sul Cancro, IST, Viale Benedelto XV no. 10, 16132 Genova, Italy |
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Abstract: | Summary Human epidermal keratinocytes grown in culture and at different stages of differentiation are shown to be viably separated by elutriation. A specific fraction enriched in melanocytes was obtained. Elutriation of cells obtained fromin vitro cultured epithlium could prove useful in studies concerning the biochemistry and molecular markers of cells isolated from normal epithelium and from different pathologies. |
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