Some properties of the amine oxidase in Vicia faba seedlings |
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| Authors: | Matsuda Hitoshi; Suzuki Yonezo |
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| Institution: | 1 Science Education Institute of Osaka Prefecture Karitacho, Sumiyoshi-ku, Osaka 558, Japan
2 Department of Biology, Faculty of Science, Osaka City University Sumiyoshi-ku, Osaka 558, Japan |
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| Abstract: | Growth of the Vicia faba seedling is accompanied by a rapid15-day increase in amine oxidase activity of the apical parts.Cotyledons and roots were found to be devoid of activity. Thepartially purified enzyme from leaves readily oxidized putrescine,cadaverine, agmatine and spermidine, while dopamine (3-hydroxytyramine)and L- and D-lysine were oxidized more slowly. The Km valueswere 1.9?10–3 M for cadaverine, 3.7?10–5 M for putrescine,7.8?10–4 M for spermidine, and 5.9?10–3 M for dopamine.Carbonyl reagents and copper-binding agents were effective inhibitorsof Vicia faba amine oxidase. The diethyldithiocarbamate-treatedenzyme could be reactivated specifically by cupric copper. (Received May 25, 1977; ) |
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