Metagenomic analysis for the microbial consortium of anaerobic CO oxidizers |
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| Authors: | Ying Guo Jingliang Xu Zhenhong Yuan Xiekun Li Weizheng Zhou Huijuan Xu Cuiyi Liang Yu Zhang Xinshu Zhuang |
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| Affiliation: | 1.Key Laboratory of Renewable Energy, Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou, 510640, China;2.Graduate University of Chinese Academy of Sciences, Beijing, 100049, China |
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| Abstract: | Metagenomics analysis has been applied to identify the dominant anaerobic microbial consortium of the carbon monoxide (CO) oxidizers in anaerobic sludge. Reads from the hypervariable V6 region in the bacterial 16s rDNA were aligned and finally clustered into operational taxonomic units (OTUs). The OTUs from different stages in anaerobic CO condition were classified. Alphaproteobacteria, clostridia, betaproteobacteria and actinobacteria were the most abundant groups, while alphaproteobacteria, betaproteobacteria and actinobacteria were variable groups. CO consumption and production efficiency of the microbial consortium were studied. Semi-continuous trials showed that these anaerobic CO oxidizers formed a stable microbial community, and the CO conversion rate was at over 84%, with the highest CO consumption activity of 28.9 mmol CO/g VSS●day and methane production activity at 7.6 mmol CH4/g VSS●day during six cycles. |
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