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Relationship between selected morphological, anatomical and cytological characteristics of leaves and the level of tolerance to herbicides in strawberry cultivars
Authors:Jerzy Lisek  Hanna Habdas  Stanisław W Gawroński
Institution:(1) Research Institute of Pomology and Floriculture, Pomologiczna 18, 96-100 Skierniewice;(2) Research Institute of Vegetable Crops, Konstytucji 3 maja 1/3, 96-100 Skierniewice;(3) Agricultural University, Nowoursynowska 166, 02-766 Warsaw
Abstract:Foliar application of a mixture of herbicides containing phenmedipham, desmedipham and ethofumesate to the plants of nine strawberry cultivars revealed that there were differences in the level of plant tolerance to the applied chemicals. Light, polarized light and scanning electron microscopy were used to explain differences in tolerance to herbicides. The surface of strawberry leaves and cells was examined for stomata, hairs, trichomes, surface structures, cucticle, vacuole and oxalate crystals. The thicker the cuticle on the adaxial leaf surface, the thicker the layer of epicuticular waxes, greater number of large vacuoles and greater number of calcium oxalate crystals in epidermis cells were characteristic for cultivars with very good tolerance to herbicides. The cracking of epicuticular waxes layer was typical to cultivars with respectively low tolerance to herbicides.
Keywords:calcium oxalate crystals  cuticle  epicuticular waxes  herbicides  stomata  strawberries  vacuole
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