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Expression and Localization of Lung Surfactant Proteins in Human Testis
Authors:Stephanie Beileke  Horst Claassen  Walter Wagner  Cord Matthies  Christian Ruf  Arndt Hartmann  Fabian Garreis  Friedrich Paulsen  Martin Schicht  Lars Br?uer
Affiliation:1. Institute of Anatomy II, Friedrich-Alexander-University Erlangen-Nürnberg, Erlangen, Germany.; 2. Institute of Anatomy and Cell Biology, Martin-Luther-University Halle-Wittenberg, Halle (Saale), Germany.; 3. Federal Armed Forces Hospital Hamburg, Department of Urology, Hamburg, Germany.; 4. Institute of Pathology, University Hospital Erlangen, Erlangen, Germany.; University of Nevada School of Medicine, UNITED STATES,
Abstract:

Background

Surfactant proteins (SPs) have been described in various tissues and fluids including tissues of the nasolacrimal apparatus, airways and digestive tract. Human testis have a glandular function as a part of the reproductive and the endocrine system, but no data are available on SPs in human testis and prostate under healthy and pathologic conditions.

Objective

The aim of the study was the detection and characterization of the surfactant proteins A, B, C and D (SP-A, SP-B, SP-C, SP-D) in human testis. Additionally tissue samples affected by testicular cancer were investigated.

Results

Surfactant proteins A, B, C and D were detected using RT-PCR in healthy testis. By means of Western blot analysis, these SPs were detected at the protein level in normal testis, seminoma and seminal fluid, but not in spermatozoa. Expression of SPs was weaker in seminoma compared to normal testicular tissue. SPs were localized in combination with vimentin immunohistochemically in cells of Sertoli and Leydig.

Conclusion

Surfactant proteins seem to be inherent part of the human testis. By means of physicochemical properties the proteins appear to play a role during immunological and rheological process of the testicular tissue. The presence of SP-B and SP-C in cells of Sertoli correlates with their function of fluid secretion and may support transportation of spermatozoa. In seminoma the expression of all SP''s was generally weaker compared to normal germ cells. This could lead to a reduction of immunomodulatory and rheology processes in the germ cell tumor.
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