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| 水稻、大麦幼苗胚乳中的苯丙氨酸解氨酶调节因子(PAL-R) | |
| 引用本文： | 薛应龙,欧阳光察,应初衍,迟小愿. 水稻、大麦幼苗胚乳中的苯丙氨酸解氨酶调节因子(PAL-R)[J]. 植物生理与分子生物学学报, 1992, 0(1) |
| 作者姓名： | 薛应龙 欧阳光察 应初衍 迟小愿 |
| 作者单位： | 复旦大学生命科学学院生物化学系，复旦大学生命科学学院生物化学系，复旦大学生命科学学院生物化学系，复旦大学生命科学学院生物化学系 上海 200433，上海 200433，上海 200433，上海 200433 |
| 基金项目： | 国家自然科学基金，国家教委博士点基金 |
| 摘 要： | 从萌发的水稻、大麦胚乳中,通过85％硫酸铵盐析、DE52纤维素和Sephadex G100柱层析,获得部分纯化的苯丙氨酸解氨酶调节因子(PAL—R),研究其基本性质,及体外PAL-R对PAL和PAL-I的影响,表明它们之间存在着相互作用。 |
| 关 键 词： | 苯丙氨酸解氨酶调节因子 水稻 大麦 胚乳 |
Studies on Regulating Factors of Phenylalanine Ammonia-Lyase (PAL-R) in Rice and Barley |
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| XUE Ying-Long,OUYANG Guang-Cha,YING Chu-Yan and CHI Xiao Yuan. Studies on Regulating Factors of Phenylalanine Ammonia-Lyase (PAL-R) in Rice and Barley[J]. Journal Of Plant Physiology and Molecular Biology, 1992, 0(1) | |
| Authors: | XUE Ying-Long OUYANG Guang-Cha YING Chu-Yan CHI Xiao Yuan |
| Abstract: | We had previously demostrated that the endosperm plays an impor tant role in PAL activity in vivo. Furthermore, a regulating factor of phenylalanine ammonia-lyase (PALR) has been isolated from the endosperm of rice and barley during germination and partially purified by ammonium sulphate fractionation, chromatography on DEAE-cellulose, sephadex G 100 gel filtration, and thePAL-R activity was found to be higher in rice endosperm than in barley endosperm (Fig. 1).Some characteristics of PAL-R from the endosperm of both rice and barley have been investigated, The results showed that the PAL-R can increase only the activity of PAL from the same species (Table 2). In vitro, the inhibiting effect of PAL-I on PAL was reduced when PAL-R was added to the medium (Fig. 3, Table 4), indicating that the PAL-R can not only activate PAL but also protect PAL from PAL-I's inhibition. |
| Keywords: | PAL-R rice barley endosperm |
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