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Generation of monoclonal antibodies detecting specific epitopes in olfactory and respiratory epithelia
Authors:Jörg Strotmann  Heinz Breer
Affiliation:(1) Institut für Zoophysiologie, Universität Stuttgart-Hohenheim, Garbenstrasse 30, W-7000 Stuttgart, Federal Republic of Germany
Abstract:
Summary Two panels of monoclonal antibodies have been generated, each panel having a distinct specificity for antigens located in the ciliary zone of either the olfactory or respiratory epithelium of rats. Tissue specificity was confirmed in enzyme-linked immunosorbent assays on membrane fractions from various tissues. During ontogeny, the expression of olfactory-specific antigens preceeds that of respiratory-specific antigens; this observation correlates with differences in the genesis of the respective cilia type and confirms that different molecular entities are recognized. A spatial segregation of immunoreactivity in the chemosensory epithelium was observed for one of the olfactory-specific monoclonal antibodies; negative zones were located in the dorsal recess of the nasal cavity and on the tips of the turbinates. Olfactory-specific antibodies reacted with distinct polypeptide bands on Western blots from olfactory ciliary preparations.
Keywords:Monoclonal antibodies  Immunocytochemistry  Olfactory epithelium  Respiratory epithelium  Antigens  (Rat Wistar)  (Mouse BALB/c)
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