| 产氨基酰化酶米曲霉高产菌株的选育 |
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| 引用本文： | 曹军卫 高春东. 产氨基酰化酶米曲霉高产菌株的选育[J]. 氨基酸和生物资源, 1999, 21(3): 20-22 |
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| 作者姓名： | 曹军卫 高春东 |
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| 作者单位： | 武汉大学生命科学学院!武汉430072 |
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| 摘 要： | 采用亚硝基瓜抑菌圈法对米曲霉菌株3042,AF92011,AF93018,AF93020,AF93022,AF93332进行诱变处理,从诱变菌株中筛选到几个高产氨基酰化酶菌株(3042-5,AF93020-2,AF93020-6和AF93022-5),对DL-蛋氨酸的拆分活力分别比出发菌株提高34.1%、157.6%、152.4%和145.4%,其中米曲霉菌株3042的诱变菌株3042-5最高酶活力达203.1U/ml
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| 关 键 词： | 米曲霉 氨基酰化酶 诱变 |
High-Producers of Aminoacylase selected from mutants of Aspergillus oryzae |
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| Cao Junwei Gao Chundong Liu Zhijian. High-Producers of Aminoacylase selected from mutants of Aspergillus oryzae[J]. Amino Acids & Biotic Resources, 1999, 21(3): 20-22 |
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| Authors: | Cao Junwei Gao Chundong Liu Zhijian |
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| Abstract: | Several strains of A. oryzae (3042,AF92011,AF930`8,AF93020,AF93022 and AF93332) were mutated by 1 methy 3 nitro 1 nitrosoguanidine. High producers of aminoacylase (3042 5, AF93020 2,AF93020 6和AF93022 5) were selected from these mutants. The Aminoacylase activities for resolving DL methioninewere were 34.1%, 157.6%,152.4% and 145.4% higher than that produced by wild type strains, respectively. Enzyme activity of the mutant A. oryzae 3042 5 from A. oryzae 3042 could reach to 203.1U/ml. |
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| Keywords: | A. oryzae Aminoacylase mutate |
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