Organization and expression of mouse Hox3 cluster genes |
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Authors: | Niels Abrahamsen Alberto Martinez Torben Kjær Leif Søndergaard and Mary Bownes |
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Institution: | (1) Developmental Biology Center, Institute of Cell and Molecular Biology, Division of Biological Sciences, University of Edinburgh, EH9 3JR Edinburgh, Scotland;(2) Institute of Genetics, University of Copenhagen, DK-1353, Denmark |
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Abstract: | Summary The three yolk protein genes (yp) of Drosophila melanogaster are transcribed in a sex- and tissue-limited fashion. We have searched for cis-regulatory sequences in regions flanking yp1 and yp2 to identify the elements that confer female-specific expression in the fat body. One such 127 by element has previously been identified in this region. We show here the existence of two additional regions which confer female fat body-specific expression on an Adh reporter gene and on the native yp2 gene, respectively. This suggests some redundancy in the regulation of expression of the yp genes. Computer searches for putative binding sites for the DSX protein, which regulates sex-specific expression of the yp genes, revealed several such sites in our constructs. However, the significance of these is unclear since many such sites also occur in genes which one would not expect to be regulated in a sex-specific manner (e.g. Adh, Actin 5C). We suggest that DSX acts in concert with other proteins to mediate sex- and tissue-specific expression of the yp genes. |
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Keywords: | Yolk protein genes dsx Sex determination Gene regulation Enhancer |
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