Plant regeneration from protoplasts isolated from cotyledons of Sesbania bispinosa |
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| Authors: | Zhao Yan-Xiu Philip J C Harris Yao Dun-Yi |
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| Institution: | (1) Department of Biology, Shandong Teachers' University, Jinan, China;(2) Division of Biological Sciences, Conventry University, CVI 5FB Coventry, UK |
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| Abstract: | Protoplasts were isolated from cotyledons of Sesbania bispinosa (Jacq.) W.F. Wight. In a liquid-over-agar culture system with Murashige and Skoog (MS) medium supplemented with 1 mg l-1 2,4-dichlorophenoxyacetic acid (2,4-d, 2 mg l-1 benzyladenine (BA), 1 mg l-1 glutamine and 0.5 and formed callus. The first division occurred after 3–4 days. Callus formed from the protoplasts differentiated shoots by organogenesis on MS medium with 1 mg l-1 indolebutyric acid (IBA) and 1 mg l-1 BA. These shoots developed into complete plantlets when excised and cultured on MS medium with 0.5 mg l-1 IBA. |
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| Keywords: | protoplast regeneration Sesbania bispinosa |
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