Methylation of nogalose during nogalomycin biosynthesis by Streptomyces nogalater Lv65

Nogalamycin is a polyketide antibiotic produced by Streptomyces nogalater Lv65. Antibiotic is glycosylated with nogalose and nogalamine sugar moieties. Bioinformatic analysis of the snogM, snogL, and snogY genes revealed that the products of these genes were involved in methylation of the nogalose moiety of nogalamycin. Disruption of the snogM, snogL, and snogY genes in the chromosome of S. nogalater Lv65 resulted in S. nogalater strains ΔsnogM, ΔsnogL, and ΔsnogY. Inactivation of the O-methyltransferase genes had no affect on the antibiotic activity and morphological features of the recombinant strains. Genetic manipulations with the snogM, snogL, and snogY genes of the nogalamycin biosynthetic gene cluster are a potentially valuable tool for generation of novel anthracycline antibiotics.