A diaphragm delivery device for flow microcalorimetry |
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| Authors: | P Picker J F Miller D W Bolen |
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| Affiliation: | 1. Department of Chemistry, University of Sherbrooke, Sherbrooke, Canada;2. Department of Chemistry and Biochemistry, Southern Illinois University, Carbondale, Illinois 62901 USA |
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| Abstract: | A diaphragm delivery device is described which allows delivery of a reagent to a flow system at a constant or variable flow rate. The device presents only a Teflon surface to the reagent and avoids direct contact with the pump. This delivery system is used to cireumvent the serious problem of reagent interaction with the working parts of pumps used in flow microcalorimetry while retaining the convenience of switching from one solution to another. The reaction of α-chymotrypsin with the nonspecific substrate 3-(2-furyl)-acryloylimidazole is used to illustrate the advantages of the diaphragm delivery device. |
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| Keywords: | To whom reprint requests should be addressed. |
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