Msp1p is an intermembrane space dynamin-related protein that mediates mitochondrial fusion in a Dnm1p-dependent manner in S. pombe |
| |
Authors: | Guillou Emmanuelle Bousquet Céline Daloyau Marlène Emorine Laurent J Belenguer Pascale |
| |
Institution: | Laboratoire de Biologie Cellulaire et Moléculaire du Contr?le de la Prolifération, Université Paul Sabatier, 118 route de Narbonne, 31062 Toulouse Cedex 04, France. |
| |
Abstract: | Mitochondrial morphology is controlled by large GTPases, such as Msp1p, whose action on mitochondrial membranes is not yet understood. The sub-mitochondrial localization of Msp1p, the subject of ongoing controversies, was found to be within the intermembrane space. Overexpression of Msp1p led to aggregation of the mitochondrial network, while its downregulation resulted in fragmentation of this network. Mutations affecting the integrity of the Msp1p GTPase function had a dominant phenotype and induced mitochondrial fragmentation followed by mitochondrial DNA loss and cell death. These effects were not observed in cells deleted for Dnm1p, an actor in mitochondrial fission, suggesting that Msp1p is involved in the fusion of mitochondria. |
| |
Keywords: | MA matrix IMS intermembrane space IM inner membrane OM outer membrane Mito-GFP mitochondrial-targeted green fluorescent protein mtDNA mitochondrial DNA DAPI 4′ 6′-diamino-2-phenylindole CAT chloroamphenicol-acetyl-transferase TEV tobacco etch virus ADOA autosomal dominant optic atrophy |
本文献已被 ScienceDirect PubMed 等数据库收录! |