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Histochemical IAA-oxidase localization in the shoot of wheat
Authors:Z. Zmrhal  Frideta Seidlová  Ivana Macháčková
Affiliation:(1) Research Institute of Crop Production, Drnovská 507, 161 06 Praha 6, Czechoslovakia;(2) Institute of Experimental Botany, Czechoslovak Academy of Sciences, Ke dvoru 15, 166 30 Praha 6, Czechoslovakia
Abstract:
A histochemical method for the determination of IAA-oxidase has been used in sections of various aerial parts of winter wheat plants. High IAA-oxidase activity was localized in the cell walls of sclerenchyma near the periphery of the stem, in the vascular bundle sheath of sclerenchyma and in xylem, both in the stem and in the leaf. The cell wall—bound IAA-oxidase activity therefore appeared in lignifying tissues. The staining was very weak or absent in the cell walls of parenchyma tissues and phloem. The positive reaction of the cytosol at the bulbous ends of guard cells and in the leaf primordia is presumed to be due to cytosolic IAA-oxidase. These results are discussed in relation to peroxidase localization and to our previousin vitro studies.
Keywords:
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