The effects of various nutritional supplements on the growth,migration and differentiation ofxenopus laevis neural crest cells in vitro |
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Authors: | H Christopher Wilson Nadine C Milos |
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Institution: | (1) Department of Anatomy, University of Alberta, 5-14 Medical Sciences Building, T6G 2H7 Edmonton, Alberta, Canada |
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Abstract: | Summary This study investigates the nutritional requirements ofXenopus laevis neural crest cells and melanophores developing in vitro. A comparison is made between the growth and differentiation of cells
in serum-containing medium and a chemically defined, serum-free medium that we have designed. Our chemically defined medium
is more efficient than serum-supplemented medium in promoting proliferation of these cells. Several supplements are required
to enhance culture development. These include insulin, α-melanocyte stimulating hormone, somatotropin, luteotrophic hormone,
linoleic acid, uridine, and putrescine. In addition, collagen and fibronectin provide the most conductive environment tested
for cell migration and adhesion.
This work was supported by establishment and major equipment grants from the Alberta Heritage Foundation for Medical Research
to N. C. M. Nadine C. Milos is a Heritage Medical Research Scholar of the Alberta Heritage Foundation for Medical Research. |
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Keywords: | defined medium melanophore migration neural crest |
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