Molecular biology of <Emphasis Type="Italic">Ganoderma</Emphasis> pathogenicity and diagnosis in coconut seedlings |
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Authors: | A Kandan R Radjacommare A Ramanathan T Raguchander P Balasubramanian R Samiyappan |
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Institution: | (1) Agricultural Biotechnology Research Center, Academia Sinica, Taipei, 115, Taiwan (R.O.C.);(2) Institute of Plant and Microbial Biology, Academia Sinica, Taipei, 115, Taiwan (R.O.C.);(3) Department of Plant Pathology, Centre for Plant Protection Studies, Tamil Nadu Agricultural University, Coimbatore, 641 003, India |
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Abstract: | The pathogenicity of Ganoderma boninense was tested on coconut seedlings under greenhouse conditions and infection confirmed by using immunological and molecular
diagnostic tools. Desiccation of older leaves and the emergence of sporophores were observed from pathogen-inoculated seedlings,
whereas a control seedling does not show any pathogenic symptoms. Mature sporophores were formed within 10–13 weeks after
inoculation. Polyclonal antibodies raised against mycelial proteins of Ganoderma were used for detection of Ganoderma in infected field palm and seedlings through indirect enzyme-linked immunosorbent assay technique. We adopted dot-immunobinding
assay for the detection of Ganoderma from greenhouse and field samples. Under nucleic-acid-based diagnosis, G. boninense (167 bp) was detected from artificially inoculated seedlings and infected field palms by polymerase chain reaction. Apart
from these, histopathological studies also support the Ganoderma pathogenicity in coconut seedlings. The pathogenicity test and combination of all the three diagnostic methods for Ganoderma could be highly reliable, rapid, sensitive and effective screening of resistance in planting material in the future. |
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