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| 一种提取麦红吸浆虫基因组DNA的方法 | |
| 引用本文： | 贺虹,魏琮,袁向群,袁锋. 一种提取麦红吸浆虫基因组DNA的方法[J]. 昆虫知识, 2008, 45(6) |
| 作者姓名： | 贺虹 魏琮 袁向群 袁锋 |
| 作者单位： | 1. 两北农林科技大学,陕西,杨凌,712100 2. 西北农林科技大学植保资源与病虫害治理教育部重点实验室,陕西,杨凌,712100 |
| 基金项目： | 西北农林科技大学校科研和教改项目 |
| 摘 要： | 介绍一种可从单头麦红吸浆虫Sitodiplosis mosellana(Gehin)成虫、幼虫和蛹成功地提取基因组DNA的方法。经检测提取DNA样品浓度为100～200ng/μL,纯度在1.4～1.6范围之间,以此为模板,可顺利地进行RAPD引物扩增。该提取方法简单、安全、经济,可为小型昆虫基因组DNA的提取提供参考。 |
| 关 键 词： | 麦红吸浆虫 DNA提取 小型昆虫 |
A method for extracting the geneme DNA of Sitodiplosis mosellana |
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| HE Hong,WEI Cong,YUAN Xiang-Qun,YUAN Feng. A method for extracting the geneme DNA of Sitodiplosis mosellana[J]. Entomological Knowledge, 2008, 45(6) | |
| Authors: | HE Hong WEI Cong YUAN Xiang-Qun YUAN Feng |
| Abstract: | Genome DNA was extracted successfully from a single individual of Sitodiplosis mosellana(Gehin),including adult,larva and pupa repectively.The concentration of DNA was 100～200 ng/μL,and the purity was 1.4～1.6.Experiment proved that the extracted DNA had high quality that could be used to RAPD analysis.The extraction method is simple,safe and economical,and it is helpful to the DNA extraction of small insects. |
| Keywords: | Sitodiplosis mosellana DNA extraction small insect |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |