钝顶螺旋藻富硒培养条件的优化

硒是人和动物必需的微量元素 ,补硒可以防治多种疾病。有机硒具有低毒、高生物利用度的优点 ,目前主要寄希望于生物转化的途径来获得有机硒1 ] 。植物对硒的生物有机化作用已有综述2 ] ,并开发有富硒酵母3 ] 、富硒菇类4] 、富硒大蒜、富硒黄芪、富硒西洋参、富硒麦芽、富硒茶以及富硒鸡蛋、富硒牛奶等5] 。螺旋藻是一种很有开发利用前景的藻类 ,但其含硒量极微 ,实验报道富硒螺旋藻对60 Ｃｏ γ射线胸部照射大鼠诱发肺炎和早期肺、肝纤维增生有防治作用6] 。在培养液中添加亚硒酸钠可以实现藻类对硒的富集和转化 ,而且螺旋藻对无机硒…

Optimization of Cultivation Conditions in Se-enriched Spirulina platensis

Orthogonal combination design was adopted in examining the Spirulina platensis (S.platensis) yield and the influence of four factors (Se content, Se-adding method, S content and NaHCO 3 content) on algae growth. The results showed that Se content, Se-adding method and NaHCO 3 content were key factors in cultivation conditions of Se-enriched S.platensis with the optimal combination being Se at 300mg/L, Se-adding amount equally divided into three times and NaHCO 3 at 16.8 g/L. Algae yield had a remarkable correlation with OD 560 and floating rate by linear regression analysis. There was a corresponding relationship between effects of the four factors on algae yield and on OD 560 , floating rate too. In conclusion, OD 560 and floating rate could be served as yield-forming factors.