|
|||||
|
|
Growth and pigment accumulation in nutrient-depleted Isochrysis aff. galbana T-ISO |
|
| Authors: | Kim J. M. Mulders Yannick Weesepoel Packo P. Lamers Jean-Paul Vincken Dirk E. Martens René H. Wijffels |
| Affiliation: | 1. Department of Agrotechnology and Food Sciences, Bioprocess Engineering, Wageningen University, P.O. Box 8129, 6700 EV, Wageningen, The Netherlands 3. FeyeCon Development & Implementation, Rijnkade 17a, 1382 GS, Weesp, The Netherlands 4. Bomenweg 2, 6703 HD, Wageningen, The Netherlands 2. Department of Agrotechnology and Food Sciences, Laboratory of Food Chemistry, Wageningen University, P.O. Box 8129, 6700 EV, Wageningen, The Netherlands |
| Abstract: | The effect of three different nutrient depletions (nitrogen, sulphur and magnesium) on the growth and pigment accumulation of the haptophyte Isochrysis aff. galbana (clone T-ISO) has been studied. Pigments were quantified based on RP-UHPLC-PDA-MSn analysis. All nutrient depletions led to reduced maximal biomass concentrations. Besides, all nutrient-depleted cultures accumulated 3-hydroxyechinenone. To our knowledge, this is the first time that 3-hydroxyechinenone has been found in I. aff. galbana T-ISO. Most 3-hydroxyechinenone, as well as the most echinenone and diatoxanthin, were found in the nitrogen-limited culture in which a more severe limitation resulted in higher cellular contents. Similar to accumulation of diatoxanthin, accumulation of 3-hydroxyechinenone and echinenone may be part of a global (stress) response mechanism to oversaturating light conditions. |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ | |