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Genomic Analysis of a New Mammalian Distal-less Gene: Dlx7 |
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| Authors: | Shuji Nakamura David W Stock Karen L Wydner Jacques A Bollekens Kenichi Takeshita Brian M Nagai Shigeru Chiba Toshio Kitamura Thomas M Freeland Zhiyong Zhao Jun Minowada Jeanne B Lawrence Kenneth M Weiss Frank H Ruddle |
| Institution: | aDepartment of Biology, Yale University, New Haven, Connecticut, 06511;cDepartment of Anthropology, Pennsylvania State University, University Park, Pennsylvania, 16802;dDepartment of Cell Biology, University of Massachusetts Medical School, Worcester, Massachusetts, 01655;eDivision of Molecular and Developmental Biology, Mount Sinai Hospital, Toronto, Ontario, M5G 1X5, Canada;fDepartment of Medicine, New York University Medical Center, New York, New York, 10016;gStanford University Medical School, Stanford, California, 94305;hThird Department of Medicine, University of Tokyo Faculty of Medicine, Tokyo, 113, Japan;iDNAX Research Institute, Palo Alto, California, 94304;bFujisaki Cell Center, Hayashibara Biochemical Laboratories, Okayama, 702, Japan |
| Abstract: | We have cloned a new Dlx gene (Dlx7) from human and mouse that may represent the mammalian orthologue of the newt geneNvHBox-5.The homeodomains of these genes are highly similar to all other vertebrate Dlx genes, and regions of similarity also exist between mammalian Dlx7 and a subset of vertebrate Dlx genes downstream of the homeodomain. The sequence divergence between human and mouse Dlx7 in these regions is greater than that predicted from comparisons of other vertebrate Dlx genes, however, and there is little sequence similarity upstream of the homeodomain both between these two genes and with other Dlx genes. We present evidence for alternative splicing of mouseDlx7upstream of the homeodomain that may account for some of this divergence. We have mapped humanDLX7distal to the 5′ end of the HOXB cluster at an estimated distance of between 1 and 2 Mb by FISH. Both the human and the mouse Dlx7 are shown to be closely linked to Dlx3 in a convergently transcribed orientation. These mapping results support the possibility that vertebrate distal-less genes have been duplicated in concert with the Hox clusters. |
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