Comparative studies of the interaction of human and bovine platelet factor 4 with heparin using histidine NMR resonances as spectroscopic probes |
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Authors: | Christopher J Talpas and Lana Lee |
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Institution: | (1) Department of Chemistry and Biochemistry, University of Windsor, N9B 3P4 Windsor, Ontario, Canada |
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Abstract: | ThepK
a
values of His-38 and His-50 of the heparin-binding protein, bovine platelet factor 4, are 5.6 and 6.5, respectively, as determined by1H NMR spectroscopy. The1H NMR resonance of His-38 of bovine platelet factor 4 which exhibits the lowerpK
a
value is perturbed upon heparin binding to a greater degree than the resonance of His-50. Human platelet factor 4 contains the homologous residues His-23 and His-35. ThepK
a
values of the two histidine residues of human platelet factor 4 are 5.3 and 6.4. The1H NMR resonance of the histidine of human platelet factor 4 exhibiting the lowerpK
a
value also is perturbed upon heparin binding to a greater degree than the histidine resonance exhibiting the higherpK
a
, thereby suggesting comparable heparin-protein interactions in bovine and human platelet factor 4. |
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Keywords: | Platelet factor 4 heparin NMR histidine |
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