添加谷氨酰胺的肠内营养对急性重症胰腺炎大鼠肠上皮细胞间紧密结合蛋白的影响

目的探讨早期肠内营养中应用谷氨酰胺(glutine,Gln)对急性重症胰腺炎(severe acute pancreatitis,SAP)大鼠肠上皮细胞间紧密结合蛋白的作用。方法:雄性SD大鼠89只,随机分成对照组,SAP+PN(肠外营养)组,SAP+EN(肠内营养)组,SAP+EN+Gln,各组又分为4 d喂养组和7 d喂养组。分别在第4 d、第7 d剖杀大鼠检测各组各项指标。免疫组化法检测小肠组织中occludin蛋白的表达。结果:(1)空肠黏膜蛋白质含量测定:各EN组粘膜蛋白质含量显著高于PN组(p<0.01),EN+Gln组的4 d、7 d分别高于EN组的4 d、7 d(p<0.01)。(2)小肠粘膜occludin蛋白表达及含量测定:PN7 d、4 d组的平均灰度值均显著高于EN、EN+Gln组(p<0.01),EN4 d、7 d组平均灰度均显著高于EN+Gln组(p<0.05)。结论:肠内营养中应用谷氨酰胺能更有效增加occludin蛋白表达,改善肠上皮紧密连接,维护肠上皮屏障完整性。

Effects of Glutamine in Enteral Nutrition on Intestinal Mucosal Tight Junction Protein in Rats with Severe Acute Pancreatitis

To study the effect of EN+Gln on integral membrane occludin protein localizing at tight junction of intestinal epithelial cells in SAP rat,Eighty-nine Sprague-Dawley rats were randomly assigned into control group,SAP+parenteral nutrition(PN) group,SAP+enteral nutriton(EN),and SAP+EN+Gln(glutamine)group.Each group was divided into four-day and seven-day groups again.Each index was tested on the 4th day or the 7th day.The expression of occludin protein was determined by SP immunchistochemical technique.Resluts:(1)The protein contents of intestinal epithelial: The protein contents of every EN groups was higher than PN groups(p<0.01),4d、7d-EN+Gln groups higher than 4d 7d-EN groups(p<0.01).(2) The expression level of occludin protein in 7d and 4d-PN groups was significantly higher than EN、EN+Gln group respectively(p<0.01),4d and 7d-EN was higher than EN+Gln groups(p<0.01).EN+Gln increased the expression of occludin protein in intestinal tight junction,improved intestinal tight junction,protecting gut barrier integrality.