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Detection of 1-O-malylglucose: pelargonidin 3-O-glucose-6'-O-malyltransferase activity in carnation (Dianthus caryophyllus) |
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| Authors: | Abe Yutaka Tera Masayuki Sasaki Nobuhiro Okamura Masachika Umemoto Naoyuki Momose Masaki Kawahara Nobuo Kamakura Hiroyuki Goda Yukihiro Nagasawa Kazuo Ozeki Yoshihiro |
| Institution: | a Department of Biotechnology and Life Science, Faculty of Engineering, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei, Tokyo 184-8588, Japan b Plant Research Center, Kirin Agribio Co. Ltd., 3377 Sarutsuka Soutome, Sakura, Tochigi 329-1414, Japan c Central Laboratories for Frontier Technology, Kirin Holdings Co. Ltd., 3377 Sarutsuka Soutome, Sakura, Tochigi 329-1414, Japan d National Institute of Health and Sciences, 1-18-1, Kamiyoga, Setagaya-ku, Tokyo 158-8501, Japan |
| Abstract: | Carnations have anthocyanins acylated with malate. Although anthocyanin acyltransferases have been reported in several plant species, anthocyanin malyltransferase (AMalT) activity in carnation has not been identified. Here, an acyl donor substance of AMalT, 1-O-β-d-malylglucose, was extracted and partially purified from the petals of carnation. This was synthesized chemically to analyze AMalT activity in a crude extract from carnation. Changes in the AMalT activity showed close correlation to the accumulation of pelargonidin 3-malylglucoside (Pel 3-malGlc) during the development of red petals of carnation, but neither AMalT activity nor Pel 3-malGlc accumulation was detectable in roots, stems and leaves. |
| Keywords: | Acyltransferase Anthocyanin Carnation Malic acid Dianthus caryophyllus |
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