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Immobilization of chitosan-modified invertase on alginate-coated chitin support via polyelectrolyte complex formation |
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| Institution: | 1. Department of Biomedical Engineering, Columbia University, New York, NY, USA;2. Department of Radiology, Columbia University, New York, NY, USA;1. Environmental Microbiology Laboratory, Environmental Toxicology Group, CSIR-Indian Institute of Toxicology Research (CSIR-IITR), Vishvigyan Bhavan 31,Mahatma Gandhi Marg, Lucknow, 226001, Uttar Pradesh, India;2. Amity Institute of Biotechnology, Amity University, Lucknow Campus, Malhaur, Near Railway Station, Gomti Nagar Extension, Lucknow, 226028, Uttar Pradesh, India |
| Abstract: | Saccharomyces cerevisiae invertase was chemically modified with chitosan and further immobilized on sodium alginate-coated chitin support. The yield of immobilized protein was determined as 85% and the enzyme retained 97% of the initial chitosan-invertase activity. The optimum temperature for invertase was increased by 10 °C and its thermostability was enhanced by about 9 °C after immobilization. The immobilized enzyme was stable against incubation in high ionic strength solutions and was four-fold more resistant to thermal treatment at 65 °C than the native counterpart. The biocatalyst prepared retained 80% of the original catalytic activity after 50 h under continuous operational regime in a packed bed reactor. |
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