Redirection of biochemical pathways for the enhancement of H2 production by Enterobacter cloacae |
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| Authors: | Narendra Kumar Agnidipta Ghosh Debabrata Das |
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| Institution: | (1) Department of Biotechnology, Indian Institute of Technology, Kharagpur, 721302, India |
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| Abstract: | Improvement in H2 production was achieved through redirection of metabolic pathways by blocking formation of alcohol and some organic acids in Enterobacter cloacae IIT-BT 08. The wild type strain was more susceptible to allyl alcohol (7 mM) and to the combined effect of NaBr and NaBrO3 (40 mM each at pH 5.5) than were double mutants, with defects in both alcohol and organic acid formation pathways, which had higher H2 yields (3.4 mol mol–1 glucose) than the wild type strain (2.1 mol mol–1 glucose). |
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| Keywords: | alcohol dehydrogenase butanediol dehydrogenase Enetrobacter cloacae H2 metabolic flux |
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