| 铜绿假单胞菌ESBLs和AmpC酶的检测及耐药性分析 |
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| 引用本文： | 毛武智,林平.铜绿假单胞菌ESBLs和AmpC酶的检测及耐药性分析[J].中国微生态学杂志,2011,23(7):647-649. |
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| 作者姓名： | 毛武智 林平 |
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| 作者单位： | 1. 临海市江南街道社区卫生服务中心,浙江,临海,317000
2. 台州学院医学院,浙江,椒江,318000 |
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| 基金项目： | 台州学院培育基金(2010PY40) |
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| 摘 要： | 目的了解铜绿假单胞菌临床分离株ESBLs和AmpC酶的产生及对常用抗菌药物敏感性,指导临床合理选用抗生素。方法常规培养分离细菌,采用VITEK-60型全自动细菌鉴定仪鉴定细菌;按NCCLS推荐的双纸片确证法和K-B纸片扩散法检测ESBLs和药敏试验;采用头孢西丁纸片扩散法筛选疑产AmpC酶阳性菌株,确诊采用三维试验。结果铜绿假单胞菌产ESBLs和AmpC酶总检出率分别为40.8%和38.2%,其中,单产AmpC酶、单产ESBLs和同产AmpC酶+ESBLs检出率分别为19.7%、26.3%和14.5%。药敏试验显示:产酶株的耐药性明显高于非产酶株,耐药现象在同产AmpC酶和ESBLs菌株中更为严重。结论台州地区临床分离的铜绿假单胞菌产ESBLs和AmpC酶菌株检出率较高。产AmpC酶和ESBLs的菌株呈高度耐药,临床上对产酶菌株引起感染的治疗应根据细菌药敏试验结果,合理选择有效的抗菌药物联合治疗,减少产酶菌株的产生和流行。
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| 关 键 词： | 铜绿假单胞菌 ESBLs AmpC酶 耐药性 |
Detection of ESBLs and AmpC enzyme in Pseudomonas aeruginosa and analysis of drug resistance |
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| MAO Wu-zhi,LIN Ping.Detection of ESBLs and AmpC enzyme in Pseudomonas aeruginosa and analysis of drug resistance[J].Chinese Journal of Microecology,2011,23(7):647-649. |
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| Authors: | MAO Wu-zhi LIN Ping |
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| Institution: | MAO Wu-zhi1,LIN Ping2(1.Healthcare Service Center of Jiangnan Street Commurity,Linhai,Linhai 317000,China,2.Medical College of Taizhou University,Jiaojiang 318000,China) |
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| Abstract: | Objective To investigate the production of ESBLs and AmpC enzymes and antimicrobial resistance in the strains of Pseudomonas aeruginosa isolated from the hospital and direct reasonable use of drugs.Method The clinically isolated P.aeruginosa strains were collected,identified by VITEK-60 type automatic bacteria identification system;ESBLs and drug resistance were detected by K-B method;the results were evaluated according to the relevant documents of NCCLS.The strains suspected producing AmpC enzymes were sc... |
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| Keywords: | Pseudomonas aeruginosa ESBLs AmpC enzyme Drug resistance |
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