| 牛源隐孢子虫分离株的分子鉴定 |
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| 引用本文： | 徐文龙,李培英,徐前明,赵长城,李锦春,王菊花.牛源隐孢子虫分离株的分子鉴定[J].中国微生态学杂志,2011,23(7):605-607,611. |
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| 作者姓名： | 徐文龙 李培英 徐前明 赵长城 李锦春 王菊花 |
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| 作者单位： | 安徽农业大学动物科技学院,安徽,合肥,230036 |
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| 基金项目： | 安徽省自然科学基金项目(070411018); 安徽省教育厅自然科学基金项目(KJ2009B027Z); 国家自然科学基金项目(31001019) |
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| 摘 要： | 目的对安徽4个牛源隐孢子虫分离株进行鉴定,旨在阐明感染牛隐孢子虫主要虫种。方法采用PCR和Nested-PCR方法分别对4个分离株18S rRNA、HSP70基因进行扩增和测序,所测定的序列经生物信息学分析它们同源性和构建种系发育进化树,以确定各分离株与其他隐孢子虫虫种之间的亲缘关系。结果 （1）它们的18S rRNA基因和HSP70基因与安氏隐孢子虫（C.andersoni）AY954887相似性可达88.3%和98.3%;（2）在遗传进化树方面,它们与AY954892（河南株）亲缘关系接近。结论此次分离的4个牛源隐孢子虫分离株均为安氏隐孢子虫（Cryptosporidium andersoni）。
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| 关 键 词： | 隐孢子虫 奶牛 种类鉴定 种系发育 |
Molecular identification of cow-derived Cryptosporidium isolates |
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| XU Wen-long,LI Pei-ying,XU Qian-ming,ZHAO Chang-cheng,LI Jin-chun,WANG Ju-hua.Molecular identification of cow-derived Cryptosporidium isolates[J].Chinese Journal of Microecology,2011,23(7):605-607,611. |
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| Authors: | XU Wen-long LI Pei-ying XU Qian-ming ZHAO Chang-cheng LI Jin-chun WANG Ju-hua |
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| Institution: | XU Wen-long,LI Pei-ying,XU Qian-ming,ZHAO Chang-cheng,LI Jin-chun,WANG Ju-hua(College of Animal Science and Technology,Anhui Agricultrual University,Hefei 230036,China) |
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| Abstract: | Objective To idertify 4 isolates of cow-derived Cryptosporidium and clarify the main species that cause the infection.Method Four Cryptosporidium isolates were identified.PCR and Nest-PCR were applied to amplify the target genes of 18S rRNA,HSP70 from four dairy farm;their products were sequenced,and the analysis of homology and establishment of their phylogeny tree were made.Result The homology in 18S rRNA and HSP70 were 88.3% and 98.3% between the four Cryptosporidium isolates and C.andersoni(AY954887);th... |
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| Keywords: | Cryptosporidium andersoni Cow Identification of variety Phylogenetic analysis |
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