| 豆豉中高效抑菌活性乳酸菌的筛选及其抑菌研究 |
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| 引用本文： | 熊骏,韩瑞娜,张忠华,殷建忠,吴少雄,柳陈坚. 豆豉中高效抑菌活性乳酸菌的筛选及其抑菌研究[J]. 中国微生态学杂志, 2011, 23(6): 485-489 |
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| 作者姓名： | 熊骏 韩瑞娜 张忠华 殷建忠 吴少雄 柳陈坚 |
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| 作者单位： | 1. 昆明理工大学生命科学与技术学院,应用微生物研究室,云南,昆明,650224
2. 昆明医学院,云南,昆明,650500 |
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| 基金项目： | 云南省科技厅面上项目(KKSA200926038) |
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| 摘 要： | 目的从云南传统发酵豆豉中分离乳酸菌,并从中筛选具有高抑菌活性的菌株,进一步研究其抑菌谱,并初步分析其抑菌机制,为新型生物防腐剂的开发提供理论依据。方法应用传统纯培养技术及spot-on-lawn法从云南传统发酵豆豉中筛选得到具有抑菌作用的乳酸菌40株,并从中筛选得到1株具有高效抑菌活性的植物乳杆菌YM-4-3菌株。通过HPLC和紫外分光对其主要抑菌物质进行分析与抑菌试验。结果 YM-4-3菌株是1株具有新型生物防腐剂应用潜力的乳酸菌菌株。结论初步推测YM-4-3菌株抑菌机制为有机酸主导,细菌素和其他化学抑菌物质协同作用。
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| 关 键 词： | 抑菌活性 乳酸菌 抑菌谱 植物乳杆菌 抑菌机制 |
Screening of Lactic acid bacteria strains with high antibacterial activity from Douchi and it's research of bacteriostatic |
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| XIONG Jun,HAN Rui-na,ZHANG Zhong-hua,YIN Jian-zhong,WU Shao-xiong,LIU Chen-jian. Screening of Lactic acid bacteria strains with high antibacterial activity from Douchi and it's research of bacteriostatic[J]. Chinese Journal of Microecology, 2011, 23(6): 485-489 |
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| Authors: | XIONG Jun HAN Rui-na ZHANG Zhong-hua YIN Jian-zhong WU Shao-xiong LIU Chen-jian |
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| Affiliation: | XIONG Jun1,HAN Rui-na1,ZHANG Zhong-hua1,YIN Jian-zhong2,WU Shao-xiong2,LIU Chen-jian1(1.Laboratory of Applied Microbiology,Faculty of Life Science and Technology,Kunming University of Science and Technology,Kunming 650224,China,2.Kunming Medical University,Kunming 650500,China) |
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| Abstract: | Objective To screen Lactic acid bacteria strains with high inhibitory activity from Yunnan Douchi,analyze its mechanism of bacteria inhibition.Method Lactic acid bacteria strains were isolated from Yunnan traditional fermented Douchi using traditional techniques of pure culture.Their antibacterial activity were assayed by spot-on-lawn method.Forty strains were identified as Lactic acid bacteria.YM-4-3 strain was identified as Lactobacillus plantarum with high antibacterial activity.Analysis and antibacteria... |
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| Keywords: | Antibacterial activity Lactic acid bacteria Antibacterial spectrum Lactobacillus plantarum Mecha-nism of inhibit bacteria |
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