| EB病毒抗体和DNA联合检测可提高儿童传染性单核细胞增多症的诊断灵敏度 |
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| 引用本文： | 严海燕,罗晓红,陈娟,曾华,庄豪,丁鹤林.EB病毒抗体和DNA联合检测可提高儿童传染性单核细胞增多症的诊断灵敏度[J].中国微生态学杂志,2011,23(6):540-542. |
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| 作者姓名： | 严海燕 罗晓红 陈娟 曾华 庄豪 丁鹤林 |
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| 作者单位： | 1. 中山大学孙逸仙纪念医院,检验科,广东,广州,510120
2. 广州医学院,广东,广州,510520 |
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| 摘 要： | 目的评估EB病毒抗体VCA-IgM、VCA-IgG、EA-IgG、EBNA-1-IgG及EBV-DNA载量检测在儿童传染性单核细胞增多症(传单)中的诊断意义。方法用ELISA方法检测70例传单患儿和25例健康儿童血清中EBV四种抗体及PCR荧光定量法检测外周血单个核细胞EBV-DNA载量。结果传单患儿组EBV-DNA的阳性率为87.14%(61/70),对照组阳性率为8.00%(2/25),传单组与对照组EBV-DNA的阳性率比较差异有统计学意义(P<0.01)。EBV抗体检测中,传单组的VCA-IgM阳性率最高,达91.43%(64/70),对照组VCA-IgM全部阴性。传单组EB病毒VCA-IgM和EBV-DNA联合检测的阳性率97.1%。结论 EBV抗体和EBV-DNA载量检测对儿童传单的诊断有极高的价值,尤其是VCA-IgM抗体和EBV-DNA联合检测,可提高儿童传单的临床诊断的敏感性。
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| 关 键 词： | EB病毒 儿童传染性单核细胞增多症 EBV抗体 EB病毒DNA |
Combined detection of Epstein-Barr virus antibodies and DNA can improve the diagnosis sensitivity in pediatric mononucleosis |
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| YAN Hai-yan,LUO Xiao-hong,CHEN Juan,ZENG Hua,ZHUANG Hao,DING He-lin.Combined detection of Epstein-Barr virus antibodies and DNA can improve the diagnosis sensitivity in pediatric mononucleosis[J].Chinese Journal of Microecology,2011,23(6):540-542. |
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| Authors: | YAN Hai-yan LUO Xiao-hong CHEN Juan ZENG Hua ZHUANG Hao DING He-lin |
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| Institution: | YAN Hai-yan1,LUO Xiao-hong1,CHEN Juan2,ZENG Hua1,ZHUANG Hao1,DING He-lin1(1.Department of Clinical Laboratory,Sun Yat-Sen Memorial Hospital,Sun Yat-sen University,Guangzhou 510120,China,2.Guangzhou Medical University Student of 2006,Guangzhou 510520,China) |
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| Abstract: | Objective To evaluate the diagnosis sensitivity of Epstein-Barr virus(EBV)DNA and four antibodies(VCA-IgM,VCA-IgG,EA-IgG and EBNA-1-IgG) in pediatric infectious mononucleosis(IM).Method EBV-DNA of peripheral blood samples from 70 pediatric IM patients and 25 healthy children were analyzed by fluorescence quantitative polymerase chain-eaction(FQ-PCR).Four antibodies were detected by ELISA method.Result The positive rate of EBV-DNA in IM group was 87.14%(61/70) and 8.00%(2/25) in the control group;the positiv... |
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| Keywords: | Epstein-barr virus Pediatric infectious mononucleosis Epstein-barr virus antibodies Epstein-barr virus DNA |
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