Specific stabilization of DNA G-quadruplex structures with a chemically modified complementary probe |
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| Authors: | Min Liu Yaqian Liu Fan Wu Yuhao Du Xiang Zhou |
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| Affiliation: | 1. Key Laboratory of Biomedical Polymers-Ministry of Education, College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China;2. The Institute for Advanced Studies, Wuhan University, Wuhan 430072, China |
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| Abstract: | The DNA G-quadruplex is an important higher-order structure formed from guanine-rich DNA sequences. There are many molecules which can stabilize this structure. However, the selectivity of these ligands to different G-quadruplexes was not satisfactory. Herein, we designed and synthesized a chemically modified G-quadruplex probe, Razo-DNA, for the unique stabilization of the G-quadruplex. Razo-DNA consists of two fragments: The first is an organic molecular moiety which can stabilize G-quadruplex structures, and the second is a DNA molecule that is complementary with a sequence adjacent to the guanine-rich sequence of targeted DNA. Further studies showed that Razo-DNA could precisely stabilize the targeted DNA G-quadruplex structures in vitro. |
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| Keywords: | G-quadruplex In situ hybridization Gene repression Polymerase Stop Assay |
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