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Survey for Zoonotic Microsporidian Pathogens in Wild Living Urban Rooks (Corvus frugilegus)
Authors:Agnieszka Perec‐Matysiak  Maria Weso?owska  Kinga Le?niańska  Katarzyna Buńkowska‐Gawlik  Joanna Hildebrand  Marta Kicia
Institution:1. Department of Parasitology, Institute of Genetics and Microbiology, University of Wroc?aw, Wroc?aw, Poland;2. Department of Biology and Medical Parasitology, Wroc?aw Medical University, Wroc?aw, Poland
Abstract:Microsporidia are opportunistic pathogens in nature infecting all animal phyla. There is a potential risk of microsporidian spores transmission from urban rooks inhabiting some metropolitan cities to people through casual interactions. The aim of this study was to identify microsporidia species in the droppings of rooks in Wroclaw, Poland. A total of 15 collective sets of droppings were examined using nested‐PCR method. Amplification of ITS rRNA gene revealed the presence of Enterocytozoon bieneusi D, Peru 6, and Encephalitozoon hellem 1A genotypes. This study indicates that excreta of urban rooks can be an important source of human infection with these pathogens.
Keywords:Encephalitozoon spp       Enterocytozoon bieneusi     genotyping     ITS   
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