Trypanosoma cruzi and American Leishmania spp: immunocytochemical localization of a laminin-like protein in the plasma membrane |
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| Authors: | A Breta?a J L Avila M Arias-Flores M Contreras F J Tapia |
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| Affiliation: | 1. Department of Radiology and Imaging, Rush University Medical Center, 3833, 1653 W Congress Pkwy, Chicago, IL 60612;2. Cardiothoracic Imaging, Department of Radiology, UT Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX, USA;3. Department of Radiology, Mayo Clinic, 4500 San Pablo Rd, Jacksonville, FL 32224;4. Chief, Cardiothoracic and Emergency Radiology, Department of Radiology, Medical College of Wisconsin, Milwaukee, WI, USA;5. Department of Diagnostic Radiology, The University of Texas MD Anderson Cancer Center, 1515 Holcombe Blvd. Unit 1478, Houston, TX, 77030;6. Department of Medical Imaging, University of Toronto, 263 McCaul Street, 4th Floor, Toronto, ON, M5T 1W7;7. Department of Radiology, Medical University of South Carolina, Charleston, South Carolina, Department of Radiology and Radiological Sciences, Medical University of South Carolina, 96 Jonathan Lucas St. MSC 323, Charleston, SC 29425;8. Department of Radiology and Medical Imaging, University of Virginia Medical Center, 1215 Lee St., Charlottesville, VA 22903;1. Kaunas University of Technology, Radvilenu rd. 19, LT-50254 Kaunas, Lithuania;2. University of Health Sciences, A. Mickeviciaus str. 9, LT-44307 Kaunas, Lithuania;1. Microbial Genomics Laboratory, Institut Pasteur de Montevideo, Montevideo, Uruguay;2. Hospital Maciel, Montevideo, Uruguay;3. Casa de Galicia, Montevideo, Uruguay;4. Center for Integrative Biology, Universidad Mayor, Santiago de Chile, Chile;5. Wellcome Sanger Institute, Hinxton, United Kingdom;1. Fishery Resources and Engineering Postgraduate Program, State University of West Paraná, Faculdade street 645, 85903-000 Toledo, PR, Brazil;2. Department of Genetics, Federal University of Paraná, Centro Politécnico, Jardim das Américas, 81531-990 Curitiba, PR, Brazil;3. Fishery Engineering graduate course, State University of West Paraná, Faculdade street 645, 85903-000 Toledo, PR, Brazil;4. Federal University of Paraná, Dr. João Maximiano street, 86900-000 Jandaia do Sul, PR, Brazil |
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| Abstract: | Patients with Chagas' disease or different clinical forms of American cutaneous leishmaniasis have high antilaminin antibody levels. An immunogold technique employing a specific antilaminin antibody was used in the present study to determine the presence, and define the ultrastructural localization, of laminin-like molecule(s) in American Leishmania spp. and Trypanosoma cruzi. Laminin was found located specifically in T. cruzi trypomastigotes on the external surface of the plasma membrane, close to the sites where the flagellar veil attaches to the plasma membrane. Laminin immunoreactivity was rapidly lost when trypomastigotes were cultured in liquid medium and no reactivity was found in fresh epimastigotes. Promastigotes and amastigotes of American Leishmania spp. also showed a specific localization of laminin immunoreactivity, this being limited to the lips of the flagellar pocket and to the parasitic side exactly opposite to the flagellar exit. These results confirm the presence of a laminin-like molecule(s) in both trypanosomatids, the specific localization suggesting a presently unknown function for this protein. |
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