Use of Trinitrobenzene as a Nitrogen Source by Pseudomonas vesicularis Isolated from Soil |
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Authors: | Emily P Davis Ramaraj Boopathy John Manning |
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Institution: | (1) Department of Biological Sciences, University of Notre Dame, Notre Dame, IN 46556, USA , US;(2) Environmental Research Division, Building 203, Argonne National Laboratory, Argonne, IL 60439, USA , US |
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Abstract: | An aerobic Gram-negative bacterium identified as
Pseudomonas vesicularis was isolated from soil contaminated with
2,4,6-trinitrotoluene (TNT) and 1,3,5-trinitrobenzene (TNB). This bacterium
used TNB as the sole source of nitrogen. The TNB was metabolized within 80 h
of incubation. The major metabolites produced were dinitroaniline,
dinitrobenzene (DNB), nitroaniline, nitrobenzene (NB), and ammonia. The
concentrations of DNB and NB produced in the culture medium were nearly
stoichiometric. The ammonia concentration in the culture medium increased
during the course of incubation. The end product of TNB metabolism was NB,
which did not undergo further degradation even after long incubation time.
This bacterium could be used in a syntrophic culture system with other
NB-degrading bacteria to remove TNB completely from soil and water at
contaminated sites.
Received: 25 July 1996 / Accepted: 10 September 1996 |
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