Increased production of FAEEs for biodiesel with lipase enhanced Saccharomyces cerevisiae |
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Authors: | Jun-Feng Liu Kai-Li Nie Li-Hai Fan Fang Wang Tian-Wei Tan Li Deng |
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Institution: | 1. Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, PR China;2. Amoy-BUCT Industrial of Biotechnovation Institute, Amoy 361026, PR China |
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Abstract: | In this study, we expressed lipase 2 from Candida sp. 99-125 in Saccharomyces cerevisiae, and tried direct biodiesel production. Driven by 3-phosphoglycerate kinase promoter, Lip2 showed high expression level in cytoplasm. SDS-PAGE analysis confirmed the successful lipase expression with a 40 kDa molecular weight. The enzyme assay indicated that lipase 2 had a specific activity of 12.12 μmol/min/mg toward p-nitrophenyl palmitate. Gas chromatography showed that the main fatty acids of S. cerevisiae lipids were palmitoleic acid (31.79%) and oleic acid (29.84%). By three-step addition of 4% ethanol to culture broth, the yield of fatty acid ethyl esters by recombinant S. cerevisiae reached 11.4 mg/g dry cell weight. This work proposed a novel pathway for S. cerevisiae that could be applied for producing biodiesel directly. |
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Keywords: | Lipase 2 Fatty acid ethyl esters (FAEEs) |
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