Biotransformation of betulin to betulone by growing and resting cells of the actinobacterium Rhodococcus rhodochrous IEGM 66 |
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Authors: | Victoria V Grishko Ekaterina V Tarasova Irina B Ivshina |
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Institution: | 1. Laboratory of Biologically Active Compounds, Institute of Technical Chemistry, Ural Branch of the Russian Academy of Sciences, 3 Ak. Korolev Str., 614013 Perm, Russia;2. Laboratory of Alkanotrophic Microorganisms, Institute of Ecology and Genetics of Microorganisms, Ural Branch of the Russian Academy of Sciences, 13 Golev Str., 614081 Perm, Russia;3. Department of Microbiology and Immunology, Perm State National Research University, 15 Bukirev Str., 614900 Perm, Russia |
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Abstract: | The ability of Rhodococcus actinobacteria to transform betulin to betulone was proved and reported for the first time. Betulone, the product of regioselective oxidation of a 3β-hydroxyl group of betulin, is a useful intermediate in the synthesis of novel biologically active compounds. Of 56 strains of Rhodococcus tested, Rhodococcus rhodochrous IEGM 66 was selected because it had the highest betulin-transforming ability. It was shown that R. rhodochrous IEGM 66 growing cells transformed 0.5 g/L betulin to betulone with 45% conversion rate within 240 h. A substantial reduction in the time of betulin (0.5 g/L) biotransformation was achieved by using resting cells, which catalyzed the production of 75% betulone after 96 h. At higher initial betulin concentrations (1.0–3.0 g/L), resting cells catalyzed 40–60% betulone production within 24 h. |
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Keywords: | Betulin Betulone Biotransformation Regioselective oxidation |
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