Short peptide fragments with antiulcer activity from a collagen hydrolysate |
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Authors: | Yu A Zolotarev K E Badmaeva Z V Bakaeva G E Samonina G N Kopylova A K Dadayan Yu B Zverkov S K Garanin B V Vaskovsky I P Ashmarin N F Myasoedov |
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Institution: | (1) Institute of Molecular Genetics, Russian Academy of Sciences, pl. akademika Kurchatova 2, Moscow, 123182, Russia;(2) Faculty of Biology, Moscow State University, Vorob’evy gory, Moscow, 117234, Russia;(3) Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, Moscow, 117997, Russia |
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Abstract: | A peptide acidic hydrolysate of collagen (PHC) was obtained under conditions (4 N HCl) ensuring the predominant formation of short peptides, glyprolines. They were separated and their antiulcer activity was studied. Thirty individual peptides with molecular masses of 174–420 amu were isolated from the PHC by HPLC. The PHC was shown to predominantly contain 2-to 4-aa peptides, including PG, GP, and PGP. Experiments on rats demonstrated that, on intragastric administration at a dose of 1 mg/kg, PHC enhances the stability of the gastric mucosa to the action of ulcerogenic factors, such as ethanol and stress, and exhibits a protecting antiulcer effect. Even a lesser dose (0.1 mg/kg), which reduced ulcer area twofold, was effective in the stress model of ulcer formation. The intraperitoneal and intragastric administration of PHC at a dose of 1 mg/kg was found to exhibit a therapeutic effect in the acetate model of ulcer formation. |
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Keywords: | antiulcer activity glyprolines peptides stomach ulcer models |
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