Molecular and phenotypic characterization of potential plant growth-promoting <Emphasis Type="Italic">Pseudomonas</Emphasis> from rice and maize rhizospheres |
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Authors: | Phrueksa Lawongsa Nantakorn Boonkerd Sopone Wongkaew Fergal O’Gara Neung Teaumroong |
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Institution: | (1) School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima, 30000, Thailand;(2) School of Crop Production Technology, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima, 30000, Thailand;(3) Department of Microbiology, Biomerit Research Centre, National University of Ireland, Cork, Ireland |
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Abstract: | In this study, Pseudomonas species were isolated from the rhizospheres of two plant hosts: rice (Oryza sativa cultivar Pathum Thani 1) and maize (Zea mays cultivar DK888). The genotypic diversity of isolates was determined on basis of amplified rDNA restriction analysis (ARDRA).
This analysis showed that both plant varieties selected for two distinct populations of Pseudomonas. The actual biocontrol and plant promotion abilities of these strains was confirmed by bioassays on fungal (Verticillum sp., Rhizoctonia solani and Fusarium sp.) and bacterial (Ralstonia solanacearum and Bacillus subtilis) plant pathogens, as well as indole-3-acetic acid (IAA) production and carbon source utilization. There was a significant
difference between isolates from rice and maize rhizosphere in terms of biological control against R. solanacearum and B. subtilis. Interestingly, none of the pseudomonads isolated from maize rhizosphere showed antagonistic activity against R. solanacearum. This study indicated that the percentage of pseudomonad isolates obtained from rice rhizosphere which showed the ability
to produce fluorescent pigments was almost threefold higher than pseudomonad isolates obtained from maize rhizosphere. Furthermore,
the biocontrol assay results indicated that pseudomonad isolated from rice showed a higher ability to control bacterial and
fungal root pathogens than pseudomonad isolates obtained from maize. This work clearly identified a number of isolates with
potential for use as plant growth-promoting and biocontrol agents on rice and maize. |
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Keywords: | Plant growth-promoting rhizobacteria (PGPR) Pseudomonas spp Amplified rDNA restriction analysis (ARDRA) Rice Maize |
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