Identification of (S)-11-cycloheptyl-4-methylundecanoic acid in acylphosphatidylglycerol from Alicyclobacillus acidoterrestris |
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Authors: | Tomáš ?ezanka Lucie Siristova Karel Sigler |
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Institution: | a Institute of Microbiology, Academy of Sciences of the Czech Republic, Vídeňská 1083, 142 20 Prague, Czech Republic b Department of Fermentation Chemistry and Bioengineering, Institute of Chemical Technology Prague, Technická 3, 166 28 Prague, Czech Republic |
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Abstract: | A method is described for the identification of molecular species of acylphosphatidylglycerols containing a branched cyclo fatty acid ((S)-11-cycloheptyl-4-methylundecanoic acid; brc19:0) from Alicyclobacillus acidoterrestris and its identification as picolinyl ester by means of GC-MS. The combination of TLC, negative RP-HPLC-ESI-MS/MS, enzymatic hydrolysis, and GC-MS was used to identify unusual molecular species of acylphosphatidylglycerols with cyclic and branched FA. The acid, brc19:0, was also synthesized to unambiguously confirm its structure. According to feeding experiments with 13C-labeled propionate, the C3 internal unit (branched methyl) of brc19:0 is assembled from propionate and not from methionine. |
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Keywords: | Alicyclobacillus acidoterrestris (S)-11-Cycloheptyl-4-methylundecanoic acid Negative RP-HPLC-ESI-MS/MS Acylphosphatidylglycerols |
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