Enzymatic synthesis of d-xylulose 5-phosphate from hydroxypyruvate and d-glyceraldehyde-3-phosphate |
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Authors: | Olga N Solovjeva German A Kochetov |
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Institution: | aA.N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, 119992 Moscow, GSP-2, Russia |
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Abstract: | An enzymatic method for obtaining d-xylulose 5-phosphate has been developed, based on the irreversible reaction catalyzed by transketolase: hydroxypyruvate + d-glyceraldehyde-3-phosphate → d-xylulose 5-phosphate. The preparations of sodium d-xylulose 5-phosphate, obtained using this approach, were 88% pure and contained no aldehyde admixtures. |
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Keywords: | Enzymatic synthesis d-Xylulose 5-phosphate" target="_blank">d-Xylulose 5-phosphate Transketolase |
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