Plant regeneration from pea protoplasts via somatic embyogenesis

Plant regeneration via somatic embryogenesis was obtained from pea protoplasts. Strong auxins (picloram or 2.4-D) and increased osmolarity of the medium were necessary for embryo induction. Relatively high amounts of embryogenic calli could be obtained in 2 genotypes. After a period on hormone-free medium, a second induction of somatic embryos was possible. Further development of somatic embryos was accomplished on GA₃ — containing medium.Abbreviations ABA abscisic acid - BA 6-benzylaminopurine - 2.4-D 2.4-dichlorophenoxyacetic acid - GA₃ gibberellic acid - Kin kinetin - NAA naphthaleneacetic acid - Pic Picloram, 4-amino-3,5,6-trichloropicolinic acid