Crystallization and preliminary X-ray crystallographic studies of phosphoglycerate kinase from Thermus caldophilus |
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Authors: | Lee Jun Hyuck Im Young Jun Rho Seong-Hwan Park Seong Ho Kang Gil Bu Cho Su Jin Kim Tae-Yeon Shin Hyun-Jae Lee Dae-Sil Eom Soo Hyun |
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Affiliation: | Department of Life Science, Kwangju Institute of Science and Technology, Gwangju 500-712, Korea. |
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Abstract: | We report the purification and crystallization of phosphoglycerate kinase from Thermus caldophilus (Tca). The enzyme crystallizes in the P2(1)2(1)2(1) space group (cell dimensions a = 65.1, b = 71.3, c = 80.2 A), with one molecule in the asymmetric unit. A complete set of diffraction data was collected from an orthorhombic crystal up to 1.8 A resolution. |
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