Enriched gonadal migration of donor-derived gonadal primordial germ cells by immunomagnetic cell sorting in birds |
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Authors: | Kim Jin Nam Kim Mi A Park Tae Sub Kim Duk Kyung Park Hyun Jeong Ono Tamao Lim Jeong Mook Han Jae Yong |
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Institution: | School of Agricultural Biotechnology, Seoul National University, Seoul, Korea. |
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Abstract: | This study was conducted to evaluate whether immunomagnetic treatment could improve the retrieval and migration capacity of avian gonadal primordial germ cells (gPGCs) collected from gonads in 5.5-day-old chick and 5-day-old quail embryos, respectively. Collected gPGCs were loaded into a magnetic-activated cell sorter (MACS) after being conjugated with specific gPGC antibodies and either MACS-treated or non-treated cells in each species were subsequently transferred to the recipient embryos. MACS treatment significantly (P < 0.05) increased the population ratio of gPGCs in gonadal cells retrieved (0.74 to 33.4% in the chicken and 2.68 to 45.1% in the quail). This was due to decreased number of non-gPGCs in total cell population. MACS treatment further enhanced gonadal migration of gPGCs transferred in both species (10% vs. 80-85% in the chicken and 10-15% vs. 70-80% in the quail). Increase in the number of microinjected cells up to 600 cells/embryo did not eliminate such promoting effect. In conclusion, MACS treatment greatly increased the population ratio of avian gPGCs in gonadal cells, resulting improved gonadal migration in recipient embryos. |
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Keywords: | gonadal primordial germ cell (gPGC) immunomagnetic treatment chicken quail gonadal migration |
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