| 有髓轴突横断损伤后钠通道聚集状态研究 |
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| 引用本文： | 陈菁,王燕,曾琳,龙在云,陈志强.有髓轴突横断损伤后钠通道聚集状态研究[J].现代生物医学进展,2009,9(13). |
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| 作者姓名： | 陈菁 王燕 曾琳 龙在云 陈志强 |
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| 作者单位： | 第三军医大学大坪医院野战外科研究所第六研究室,创伤、烧伤与复合伤国家重点实验室,重庆,400042 |
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| 基金项目： | 国家自然科学基金资助(No.90608013) |
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| 摘 要： | 目的:研究有髓轴突横断损伤后郎飞结区钠通道聚集状态的变化.方法:用雪旺细胞-背根神经元髓鞘化共培养系统复制周围神经髓鞘形成和郎飞结发育,于髓鞘化培养基中共培养第14天用前房角切开刀造成有髓轴突横断损伤,在损伤后1、2、3、4、5、6、7、14天进行髓鞘碱性蛋白和钠通道免疫荧光染色,损伤前共培养作为对照.利用SPOT图像分析软件测量钠通道聚集簇的直径、长度和直径/长度比.结果:损伤前钠通道蛋白在有髓轴突郎飞结区形成直径/长度比略大于1的聚集簇;有髓轴灾横断损伤后钠通道蛋白沿轴突纵向扩散,钠通道聚集簇的直径/长度比逐渐减小,损伤后第14天已无法检测到钠通道表达.损伤区出现节段性脱髓鞘.结论:轴突横断损伤可造成钠通道聚集簇扩散、消失,导致郎飞结结构破坏.
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| 关 键 词： | 有髓轴突 横断损伤 钠通道聚集 郎飞结 |
Dynamics of Sodium Channels Clustering in Myelinated Axons after Transection |
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| CHEN Jing,WANG Yan,ZENG Lin,LONG Zai-yun,CHEN Zhi-qiang.Dynamics of Sodium Channels Clustering in Myelinated Axons after Transection[J].Progress in Modern Biomedicine,2009,9(13). |
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| Authors: | CHEN Jing WANG Yan ZENG Lin LONG Zai-yun CHEN Zhi-qiang |
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| Institution: | CHEN Jing,WANG Yan,ZENG Lin,LONG Zai-yun,CHEN Zhi-qiang(State Key Laboratory of Trauma,Burn and Combined Injury,Research Institute of Surgery,Daping Hospital,Third Military Medical University,Chongqing 400042,China) |
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| Abstract: | Objective: To investigate the dynamics of sodium channel clustering in the nodal region of myelinated axons after transection.Methods: Myelinating cocultures of Schwann cell and dorsal root ganglion neuron were prepared to replicate myelination and the formation of node of Ranvier in peripheral nervous system.Axonal transection was carried out on myelinated axons in cocultures maintained under conditions promoting myelination for 14 days using a goniotomy knife under microscope guidance.Immunofluorescence s... |
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| Keywords: | Myelinated axon Transection Sdium channel clustering Node of Ranvier |
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