RNA preservation of Antarctic marine invertebrates |
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Authors: | Guy Hillyard and Melody S Clark |
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Institution: | (1) British Antarctic Survey, Natural Environment Research Council, High Cross, Madingley Road, Cambridge, CB3 0ET, UK; |
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Abstract: | Fifteen species of marine invertebrate commonly occurring in the near-shore environment of Rothera base, Antarctica, were
used to test tissue sample storage protocols with regard to preservation of RNA integrity. After animal collection, the tissues
were either immediately extracted for RNA or stored at −80°C after having been, either directly flash frozen in liquid nitrogen
or preserved in a commercial RNA storage solution, for extraction in the UK. In four cases, direct flash freezing produced
enhanced RNA integrity compared with samples in the commercial storage solution. A subset of samples were further tested for
the preferred temperature of storage in the commercial reagent. RNA integrity was well preserved at both +4 and −20°C over
periods of 2 months, but degradation was rapid in tissues stored at room temperature. Eight out of the fifteen species only
produced a single ribosomal band on gel electrophoresis. This survey provides a guide for tissue transport of Polar cold water
marine invertebrates. |
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