Expression of p97/VCP and ubiquitin during postnatal development of the degenerating rat retina |
| |
Authors: | Huseyin Ortak Sevil Cayli Ufuk Tas Seda Ocakli Erkan S??üt Helin Deniz Demir |
| |
Institution: | (1) Department of Ophthalmology, Faculty of Medicine, Gaziosmanpasa University, Tokat, Turkey;(2) Department of Histology and Embryology, Faculty of Medicine, Gaziosmanpasa University, Tokat, Turkey;(3) Department of Anatomy, Faculty of Medicine, Gaziosmanpasa University, Tokat, Turkey;(4) Department of Biochemistry, Faculty of Medicine, Gaziosmanpasa University, Tokat, Turkey |
| |
Abstract: | In this study, we aimed to investigate the distribution pattern of ubiquitin and p97/VCP in the rat retina during postnatal
development. Eyeballs from 1-, 4-, 10-, 36- and 72-week-old rats were examined by immunohistochemistry, and protein colocalization
was determined by immunofluorescence microscopy. In the 1-week-old rat retina, p97/VCP was strongly expressed in the neuroblast
layer, however no ubiquitin immunoreactivity was observed. p97/VCP immunoreactivity was present in the ganglion cell layer
(GCL), inner nuclear layer (INL), outer nuclear layer (ONL), inner segment (IS) of the photoreceptor layer, and retinal pigment
epithelium in the 4- and 10-week-old rat retinas. p97/VCP immunoreactivity increased significantly in the 10-week-old rat
retinas. Ubiquitin was barely seen in the 4-week-old rat retinas, and ubiquitin expression was weak in the GCL and the IPL
of the 10-week-old rat retinas. In the 36- and 72-week-old rats, the presence of ubiquitin was remarkable in the IS, INL,
IPL and GCL, however, p97/VCP immunoreactivity was significantly decreased. Colocalization of ubiquitin and p97/VCP was also
observed in the INL, IS, GCL and ONL of 36- and 72-week-old rat retinas. Our results indicate that p97/VCP immunoreactivity
in the retina significantly decreases after rats reach 10 weeks of age, whereas ubiquitin immunoreactivity increases with
aging. These results suggest that an altered expression pattern of p97/VCP and ubiquitin in the developing rat retina may
associate with age-related retinal degeneration. |
| |
Keywords: | |
本文献已被 PubMed SpringerLink 等数据库收录! |
|